Researchers have used ancient DNA from grape seeds to trace the origins of wine-making traditions that still shape modern viticulture, finding a direct genetic thread connecting an Etruscan settlement in Italy to wine regions across Europe today.

Oya Inanli of the University of York led a team that studied 80 waterlogged grape seeds from two wells at Cetamura del Chianti, a site in Tuscany dating to around 300 BC.

The seeds span the Etruscan and Roman periods up to roughly 1200 CE. The study was published in the Journal of Archaeological Science.

More than a quarter of the seeds belonged to a single variety, identical in genetics, and maintained without change for at least 362 years. Direct radiocarbon dating confirmed this variety was present from the Etruscan period through Roman occupation.

Researchers identified it as a clonal lineage, meaning winemakers repeatedly propagated the same vine without allowing it to reproduce sexually. This practice remains common among winemakers today.

One grape variety survived unchanged for over three centuries

The DNA of the dominant variety also pointed to a significant discovery. Genetic markers associated with berry color showed a 92 percent likelihood that the clonal variety produced white grapes.

A new study decodes ancient grape DNA, tracing a single wine variety through over 362 years and connecting Roman-era viticulture to modern European winemaking traditions. pic.twitter.com/mRRzkTyCuF

— Tom Marvolo Riddle (@tom_riddle2025) June 12, 2026

This makes Cetamura del Chianti one of the earliest known sites with genetic evidence for white wine production in the pre-Roman Mediterranean. Two other seeds showed markers linked to dark berries, suggesting red wine was also part of life at the settlement.

Researchers found genetic links stretching well beyond Tuscany. A Cetamura seed closely matched a grape seed recovered from a first-century Roman farm in Mont Ferrier, France. This points to the Romans moving specific vine varieties deliberately across their empire.

A separate Cetamura seed from the transitional Etruscan-Roman period showed a sibling-level genetic relationship with a modern Hungarian variety called “Baratcsuha szurke.” That variety belongs to a broader family of old European grapes, including a vine in Slovenia said to be more than 400 years old.

Ancient grape DNA traces modern wine’s origins across Europe

The team applied multiple methods, including ancient DNA analysis, near-infrared spectroscopy, geometric morphometrics, and radiocarbon dating.

Seeds from deeper layers of the wells preserved more genetic material, pointing to stable, waterlogged conditions as a key factor in DNA survival.

The research provides concrete evidence that agricultural traditions of the Etruscans and Romans laid the groundwork for wine culture across Europe.

Researchers examining the remains of an Iron Age woman in northern Scotland have found signs that her brain may have been intentionally removed more than 2,000 years ago.

The discovery, made at a burial cairn in Sutherland, also revealed long-distance family connections, suggesting prehistoric communities maintained social and cultural ties across wide areas of Scotland.

The findings were published in the journal Antiquity and come from an investigation of two individuals buried in a low stone cairn at Loch Borralie, near the northwestern tip of mainland Scotland. The burial contained the remains of an adult woman and a juvenile boy.

Rare glimpse into Iron Age burial practices

Understanding funerary traditions in Iron Age Britain has long been difficult because human remains rarely survive. Soil conditions often destroy bone over time. North-west Scotland is one of the few regions where environmental conditions have preserved skeletal remains, offering researchers a rare opportunity to study how ancient communities treated their dead.

Researchers from the United Kingdom and the United States examined the remains to determine who the individuals were, where they came from, and whether they were related to each other or other groups in the region.

“We knew that in the north-west of Scotland, including the Northern and Western Isles, the circulation and deposition of human remains were particularly prominent,” said lead author Dr. Laura Castells Navarro of the University of York.

Evidence points to deliberate brain removal

To investigate the burial, researchers combined osteological analysis, isotope testing, and ancient DNA studies.

The most striking discovery involved the adult woman. Researchers found fine cut marks on the inside of her skull and identified unusual modifications to several long bones, including the upper arm, forearm, and thigh bones. The bones had been shaped so that their ends tapered into points.

According to the researchers, the evidence suggests the woman’s brain was intentionally removed after death, and some of her bones were deliberately carved before burial.

The purpose of these actions remains unclear. However, researchers believe the treatment may indicate that the woman held a special place within her community.

“The motivation behind the extensive manipulation of the skeletal remains of Individual 1 is very difficult to interpret,” Castells Navarro said. “However, the care with which she was reassembled and deposited in the cairn possibly suggests she commanded a level of reverence and respect by her community.”

DNA reveals connections across Scotland

The study also uncovered evidence of extensive mobility among Iron Age communities.

Isotope analysis indicated that both individuals likely spent their childhoods about 80 kilometers southeast of Loch Borralie. Ancient DNA revealed genetic links to people living in Orkney, roughly 175 kilometers northeast of the site, and Applecross, around 225 kilometers to the southwest.

Researchers also determined that the woman and the boy were closely related, most likely maternal second cousins.

The findings suggest that family groups moved regularly around Scotland’s northern coast and islands while maintaining relationships across considerable distances.

“More broadly, our research shows that prehistoric maritime communities periodically moved around the north coast and Northern Isles of Scotland, possibly in small groups,” Castells Navarro said. “This movement allowed for the spread and maintenance of cultural practices and traditions.”

New clues to prehistoric social networks

Taken together, the evidence of possible brain removal, deliberate bone modification, and long-distance family connections points to a complex society linked by shared traditions and social networks.

While researchers cannot determine exactly why the woman’s remains were altered, the burial suggests that some Iron Age communities maintained ongoing relationships with the dead and practiced funerary rituals that extended far beyond simple burial.

The findings provide a rare glimpse into beliefs and customs that shaped life in prehistoric Scotland more than two millennia ago.

Ancient DNA from the bones of Iron Age Iberians shows that these people held onto their genetic roots despite centuries of Greek, Phoenician, and Carthaginian influence along the Mediterranean coast.

A study published in iScience traced genetic changes across northeastern Spain from 775 BC to 50 CE, marking the first time researchers have systematically analyzed Iberian communities from the early Iron Age all the way through Roman conquest.

Daniel R. Cuesta-Aguirre of the Faculty of Biosciences at Universitat Autònoma de Barcelona led the research. Because Iberian adults were cremated, their remains left no usable DNA.

Researchers instead turned to newborn burials found beneath house floors and work areas at three sites: the Vilars fortress, Sant Miquel d’Olèrdola, and El Camp de les Lloses. Of 54 newborns examined, 22 yielded genome-wide data, and nine more contributed mitochondrial markers.

From fortress to Roman hub: three windows into the Iberian past

The three sites covered distinct phases of Iberian history. The Vilars fortress, home to the Ilergetae tribe, was occupied from the 8th to the 3rd century BC and captured the shift from pre-Iberian to full Iberian culture.

Sant Miquel d’Olèrdola, linked to the Cessetani tribe and active from the 4th to the 2nd century BC, reflected an established community with active trade connections.

El Camp de les Lloses, tied to the Ausetani tribe and dated from the late 2nd century BC to the 1st century CE, documented the period of growing Roman presence.

Genetic analysis placed most individuals from the Vilars fortress and Sant Miquel d’Olèrdola firmly within the local Bronze Age population. Their ancestry combined Western Hunter-Gatherers, Anatolian Neolithic farmers, and Steppe-related groups.

Steppe-related ancestry was somewhat higher in Iron Age individuals than in earlier Bronze Age populations from the same region, suggesting gradual internal shifts rather than large outside migrations.

Iberian DNA and Greek and Mediterranean influence

The DNA record of Iberians showed only limited traces of Greek and other Mediterranean genetic influence before Roman arrival. A few individuals carried ancestry linked to North Africa or southwestern Asia, but these were not widespread.

One newborn at Sant Miquel d’Olèrdola had a maternal lineage associated with North Africa. Researchers interpreted this as possibly reflecting a child born to a local parent and someone with Punic or Carthaginian ancestry, consistent with the large amounts of Punic pottery recovered at the same site.

The study also settled a long-standing archaeological debate. Two newborns at Sant Miquel d’Olèrdola, OLE04 and OLE06, were buried with their legs intermingled and no sediment between them, leading earlier researchers to believe they were twins.

DNA analysis proved they were completely unrelated and even carried different maternal haplogroups.

Roman military activity brought new genes to Iberian settlements

At El Camp de les Lloses, the pattern shifted noticeably. Five of the nine individuals showed clear genetic differences from earlier Iberian populations, carrying North African or Iranian Neolithic ancestry absent in previous generations.

Researchers connected this directly to Roman military and commercial activity, as the site served as a logistical hub tied to Roman infrastructure.

Taken together, the results point to a population that stayed genetically stable for centuries before Roman expansion fundamentally reshaped its makeup.